Abstract
This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60 min of exposure time. At 16.8 microM concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100 microM, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a gamma-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signal-regulated kinase mediated and gamma-aminobutyric acid-A independent pathway.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bicuculline / pharmacology
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Blotting, Western
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Cell Death
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Early Growth Response Protein 1 / biosynthesis
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Early Growth Response Protein 1 / genetics*
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Enzyme Inhibitors / pharmacology
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Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
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Extracellular Signal-Regulated MAP Kinases / metabolism*
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Flavonoids / pharmacology
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GABA Agents / pharmacology*
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GABA Antagonists / pharmacology
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GABA-A Receptor Antagonists
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Genes, fos / drug effects*
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Mice
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Neurons / drug effects*
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Neurons / metabolism
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Polymerase Chain Reaction
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Propofol / pharmacology*
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Proto-Oncogene Proteins c-fos / biosynthesis*
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Transcription, Genetic / drug effects
Substances
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Early Growth Response Protein 1
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Egr1 protein, mouse
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Enzyme Inhibitors
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Flavonoids
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GABA Agents
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GABA Antagonists
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GABA-A Receptor Antagonists
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Proto-Oncogene Proteins c-fos
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Extracellular Signal-Regulated MAP Kinases
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
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Bicuculline
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Propofol