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. 2009 Feb;72(2):325-31.
doi: 10.4315/0362-028x-72.2.325.

Antipathogenic Properties of Green Tea Polyphenol Epigallocatechin Gallate at Concentrations Below the MIC Against Enterohemorrhagic Escherichia Coli O157:H7

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Antipathogenic Properties of Green Tea Polyphenol Epigallocatechin Gallate at Concentrations Below the MIC Against Enterohemorrhagic Escherichia Coli O157:H7

Kang-Mu Lee et al. J Food Prot. .

Abstract

The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 microg/ml, which are lower than the MIC (539 +/- 22 microg/ml). At 25 microg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 microg/ml. Our results suggest that EGCG at concentrations below itsMIC has significant antipathogenic effects against E. coli O157:H7.

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