Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade

Biochem Biophys Res Commun. 2009 Mar 27;381(1):75-80. doi: 10.1016/j.bbrc.2009.02.011. Epub 2009 Feb 10.


Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBPalpha and PPARgamma but not of C/EBPbeta. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPbeta induces PPARgamma and C/EBPalpha expressions, by controlling the MAPK pathway.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / enzymology
  • Adipocytes / physiology*
  • Adipogenesis*
  • Animals
  • Cell Proliferation
  • Gene Knockdown Techniques
  • MAP Kinase Signaling System*
  • Mice
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology*
  • RNA Interference


  • Nerve Tissue Proteins
  • neudesin protein, mouse