Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats

Free Radic Res. 2009 May;43(5):478-84. doi: 10.1080/10715760902870603.


Hydrogen gas was reported to reduce reactive oxygen species and alleviate cerebral, myocardial and hepatic ischemia/reperfusion (I/R) injuries. This paper studied the effect of hydrogen-rich saline, which was easier for clinical application, on the intestinal I/R injury. Model of intestinal I/R injury was induced in male Sprague-Dawley rats. Physiological saline, hydrogen-rich saline or nitrogen-rich saline (5 ml/kg) was administered via intravenous infusion at 10 min before reperfusion, respectively. The intestine damage was detected microscopically and was assessed by Chiu score system after I/R injury. In addition, serum DAO activity, TNF-alpha, IL-1beta and IL-6 levels, tissue MDA, protein carbonyl and MPO activity were all increased significantly by I/R injury. Hydrogen-rich saline reduced these markers and relieved morphological intestinal injury, while no significant reduction was observed in the nitrogen-rich saline-treated animals. In conclusion, hydrogen-rich saline protected the small intestine against I/R injury, possibly by reduction of inflammation and oxidative stress.

MeSH terms

  • Animals
  • Cytokines / blood
  • Hydrogen / administration & dosage*
  • Inflammation / prevention & control
  • Inflammation Mediators / blood
  • Infusions, Intravenous
  • Intestinal Mucosa / metabolism
  • Intestines / blood supply*
  • Intestines / injuries*
  • Intestines / pathology
  • Male
  • Malondialdehyde / metabolism
  • Neutrophils / drug effects
  • Neutrophils / pathology
  • Nitrogen / administration & dosage
  • Oxidative Stress / drug effects
  • Peroxidase / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Reperfusion Injury / prevention & control*
  • Sodium Chloride / administration & dosage


  • Cytokines
  • Inflammation Mediators
  • Sodium Chloride
  • Malondialdehyde
  • Hydrogen
  • Peroxidase
  • Nitrogen