Abstract
The nonhistone chromatin protein, C-14, was extracted from chromatin of Novikoff hepatoma ascites cells and isolated in high purity as shown by its migration as a single dense spot on two-dimensional polyacrylamide gels. Its mobility on sodium dodecyl sulfate gels is consistent with a molecular weight of approximately 70 000. The amino acid composition shows that protein C-14 has an acidic:basic amino acid ratio of 1.8. Its amino terminal amino acid is lysine. Protein C-14 stimulated the incorporation of [3H]UMP into RNA by approximately 30% when added to naked DNA and homologous RNA polymerase I. A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an E. coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acids / analysis
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Carcinoma, Hepatocellular / metabolism
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Cell Nucleolus / analysis
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Cell Nucleus / analysis
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Chemical Phenomena
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Chemistry
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Chromatin / isolation & purification
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Chromatography, Gel
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Chromosomal Proteins, Non-Histone / isolation & purification*
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Chromosomal Proteins, Non-Histone / pharmacology
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Chromosomal Proteins, Non-Histone / physiology
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / enzymology
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In Vitro Techniques
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Liver Neoplasms / metabolism
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Molecular Weight
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Neoplasms, Experimental / metabolism
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RNA Polymerase I / isolation & purification
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RNA, Neoplasm / biosynthesis*
Substances
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Amino Acids
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Chromatin
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Chromosomal Proteins, Non-Histone
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RNA, Neoplasm
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RNA Polymerase I