Isolation and characterization of nonhistone chromosomal protein C-14 which stimulates RNA synthesis

Biochemistry. 1977 May 31;16(11):2384-9. doi: 10.1021/bi00630a012.


The nonhistone chromatin protein, C-14, was extracted from chromatin of Novikoff hepatoma ascites cells and isolated in high purity as shown by its migration as a single dense spot on two-dimensional polyacrylamide gels. Its mobility on sodium dodecyl sulfate gels is consistent with a molecular weight of approximately 70 000. The amino acid composition shows that protein C-14 has an acidic:basic amino acid ratio of 1.8. Its amino terminal amino acid is lysine. Protein C-14 stimulated the incorporation of [3H]UMP into RNA by approximately 30% when added to naked DNA and homologous RNA polymerase I. A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an E. coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Carcinoma, Hepatocellular / metabolism
  • Cell Nucleolus / analysis
  • Cell Nucleus / analysis
  • Chemical Phenomena
  • Chemistry
  • Chromatin / isolation & purification
  • Chromatography, Gel
  • Chromosomal Proteins, Non-Histone / isolation & purification*
  • Chromosomal Proteins, Non-Histone / pharmacology
  • Chromosomal Proteins, Non-Histone / physiology
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology
  • In Vitro Techniques
  • Liver Neoplasms / metabolism
  • Molecular Weight
  • Neoplasms, Experimental / metabolism
  • RNA Polymerase I / isolation & purification
  • RNA, Neoplasm / biosynthesis*


  • Amino Acids
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • RNA, Neoplasm
  • RNA Polymerase I