Abstract
A stable cell line was developed to assess activation of rat pregnane X receptor (rPXR) by xenobiotics. Characterization revealed that the greatest response occurred with dexamethasone (6-10 fold increase) while rifampicin failed to transactivate rPXR. Rodent PXR transactivation assays can be used to understand species differences in enzyme induction.
MeSH terms
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Animal Use Alternatives / methods
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Animals
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Cell Line
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Cytochrome P-450 CYP3A / drug effects*
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Cytochrome P-450 CYP3A / metabolism
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Dexamethasone / pharmacology
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Enzyme Induction / drug effects
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Humans
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Pregnane X Receptor
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Rats
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Receptors, Steroid / drug effects*
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Receptors, Steroid / metabolism
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Rifampin / pharmacology
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Species Specificity
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Transcriptional Activation / drug effects*
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Xenobiotics / pharmacology*
Substances
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Pregnane X Receptor
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Receptors, Steroid
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Xenobiotics
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Dexamethasone
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Cytochrome P-450 CYP3A
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Rifampin