Primary human immunodeficiency virus type 1-specific CD8+ T-cell responses induced by myeloid dendritic cells

J Virol. 2009 Jun;83(12):6288-99. doi: 10.1128/JVI.02611-08. Epub 2009 Apr 8.


Induction of an antigenically broad and vigorous primary T-cell immune response by myeloid dendritic cells (DC) in blood and tissues could be important for an effective prophylactic or therapeutic vaccine to human immunodeficiency virus type 1 (HIV-1). Here we show that a primary CD8(+) T-cell response can be induced by HIV-1 peptide-loaded DC derived from blood monocytes of HIV-1-negative adults and neonates (moDC) and by Langerhans cells (LC) and interstitial, dermal-intestinal DC (idDC) derived from CD34(+) stem cells of neonatal cord blood. Optimal priming of single-cell gamma interferon (IFN-gamma) production by CD8(+) T cells required CD4(+) T cells and was broadly directed to multiple regions of Gag, Env, and Nef that corresponded to known and predicted major histocompatibility complex class I epitopes. Polyfunctional CD8(+) T-cell responses, defined as single-cell production of more than one cytokine (IFN-gamma, interleukin 2, or tumor necrosis factor alpha), chemokine (macrophage inhibitory factor 1beta), or cytotoxic degranulation marker CD107a, were primed by moDC, LC, and idDC to HIV-1 Gag and reverse transcriptase epitopes, as well as to Epstein-Barr virus and influenza A virus epitopes. Thus, three major types of blood and tissue myeloid DC targeted by HIV-1, i.e., moDC, LC, and idDC, can prime multispecific, polyfunctional CD8(+) T-cell responses to HIV-1 and other viral antigens.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / virology
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Fetal Blood / cytology
  • HIV Infections / immunology*
  • HIV-1 / immunology*
  • HLA-A Antigens / immunology
  • HLA-A2 Antigen
  • Humans
  • Infant, Newborn
  • Langerhans Cells / immunology
  • Lymphocyte Activation / immunology*
  • Myeloid Cells / immunology


  • HLA-A Antigens
  • HLA-A*02:01 antigen
  • HLA-A2 Antigen