Generation of red fluorescent protein transgenic dogs

Genesis. 2009 May;47(5):314-22. doi: 10.1002/dvg.20504.

Abstract

Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene-expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP-fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified*
  • Animals, Newborn
  • Blotting, Southern
  • Dogs / genetics*
  • Embryo Transfer
  • Embryo, Mammalian / metabolism
  • Female
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Genetic Vectors / genetics
  • Genotype
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Luminescent Proteins / genetics*
  • Luminescent Proteins / metabolism
  • Male
  • Microscopy, Fluorescence
  • Nuclear Transfer Techniques
  • Oocytes / cytology
  • Oocytes / metabolism
  • Pregnancy
  • Retroviridae / genetics
  • Transfection
  • Transgenes / genetics*

Substances

  • Luminescent Proteins
  • red fluorescent protein