Abstract
A new two-dimensional isoelectric focusing method was developed to identify the human esterase D (EsD) subunits from the homodimeric and heterodimeric forms of five EsD phenotypes. EsD polymorphism was also analyzed by one-dimensional isoelectric focusing under reducing and mild denaturing conditions to study the influence of dithiothreitol and low concentrations of urea on the focusing pattern of the EsD dimers.
MeSH terms
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Alleles
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Carboxylesterase*
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Carboxylic Ester Hydrolases / blood*
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Carboxylic Ester Hydrolases / genetics
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Chromosomes, Human, Pair 13
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Electrophoresis, Polyacrylamide Gel
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Erythrocytes / enzymology
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Genes
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Genetic Markers
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Humans
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Isoelectric Focusing / methods*
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Isoenzymes / blood*
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Isoenzymes / genetics
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Oxidation-Reduction
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Phenotype
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Protein Denaturation
Substances
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Genetic Markers
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Isoenzymes
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Carboxylic Ester Hydrolases
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Carboxylesterase
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ESD protein, human