Correlation of inhibitor of differentiation 1 expression to tumor progression, poor differentiation and aggressive behaviors in cervical carcinoma

Gynecol Oncol. 2009 Jul;114(1):89-93. doi: 10.1016/j.ygyno.2009.03.016. Epub 2009 Apr 9.


Objective: To study the correlation of inhibitor of differentiation 1 (Id-1) to tumor invasion and metastasis by examining Id-1 expression levels in different stages of cervical carcinogenesis.

Methods: Id-1 mRNA and protein expression was detected in total of 171 cervical samples including precancerous and cancerous tissues by quantitative RT-PCR (qRT-PCR) and immunohistochemistry (IHC), respectively. Twenty-five normal cervical tissues were used as a normal control. Correlation between Id-1 positive rates and expression levels to cancer progression and clinicopathologic features was statistically analyzed.

Results: A gradual increase of Id-1 protein expression associated with cervical cancer progression was detected (4%, 16%, 50% and 75.9% in normal, low squamous intraepithelial lesion (LSIL), high squamous intraepithelial lesion (HSIL) and cancer tissue, respectively, p<0.001). A similar trend of Id-1 mRNA expression was also observed (1.3, 3.4 and 10.4 fold higher than normal tissues in LSIL, HSIL and cancer tissue, respectively, p<0.001). Furthermore, the Id-1 expression level was correlated to tumor grade (p=0.005), lymph node metastasis (p=0.001), interstitial invasive (p<0.001) and tumor size (p<0.001). These results suggest that high Id-1 expression is associated with tumor growth, invasion and metastasis.

Conclusion: Id-1 expression is correlated to progression and aggressive behaviors in cervical cancer, suggesting a tumor-promoting role for Id-1 in progression of this malignancy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggression*
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / psychology
  • Carcinoma, Squamous Cell / surgery
  • Cell Differentiation
  • DNA Primers
  • Disease Progression
  • Female
  • Growth Differentiation Factor 1 / genetics*
  • Humans
  • Hysterectomy
  • Immunohistochemistry
  • Lymph Node Excision
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • RNA, Messenger / genetics
  • RNA, Neoplasm / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / pathology*
  • Uterine Cervical Neoplasms / psychology*
  • Uterine Cervical Neoplasms / surgery


  • DNA Primers
  • Growth Differentiation Factor 1
  • RNA, Messenger
  • RNA, Neoplasm