Abstract
Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin alpha 3 beta 1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesiveness
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Amino Acid Sequence
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Animals
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Antibodies / analysis*
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Camelids, New World
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Cell Adhesion
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Cell Membrane / immunology
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Enzyme-Linked Immunosorbent Assay
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Extracellular Matrix / immunology
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Fluorescent Antibody Technique
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HeLa Cells
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Humans
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Immunoglobulin Heavy Chains / chemistry
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Immunoglobulin Heavy Chains / immunology*
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Immunoglobulin Variable Region / chemistry
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Immunoglobulin Variable Region / immunology*
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Immunoprecipitation
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Integrin alpha3beta1 / chemistry
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Integrin alpha3beta1 / immunology*
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Keratinocytes / cytology
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Keratinocytes / immunology
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Microscopy, Fluorescence
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Molecular Sequence Data
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Proteomics / methods*
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Reproducibility of Results
Substances
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Antibodies
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Integrin alpha3beta1