Highly sensitive spectrofluorimetric determination of lomefloxacin in spiked human plasma, urine and pharmaceutical preparations

Eur J Med Chem. 2009 Sep;44(9):3402-5. doi: 10.1016/j.ejmech.2009.02.019. Epub 2009 Feb 21.

Abstract

A sensitive, simple and selective spectrofluorimetric method was developed for the determination of lomefloxacin in biological fluids and pharmaceutical preparations. The method is based on the reaction between the drug and 4-chloro-7-nitrobenzodioxazole in borate buffer of pH 8.5 to yield a highly fluorescent derivative that is measured at 533 nm after excitation at 433 nm. The calibration curves were linear over the concentration ranges of 12.5-625, 15-1500 and 20-2000 ng/mL for plasma, urine and standard solution, respectively. The limits of detection were 4.0 ng/mL in plasma, 5.0 ng/mL in urine and 7.0 ng/mL in standard solution. The intra-assay accuracy and precision in plasma ranged from 0.032 to 2.40% and 0.23 to 0.36%, respectively, while inter-assay accuracy and precision ranged from 0.45 to 2.10% and 0.25 to 0.38%, respectively. The intra-assay accuracy and precision estimated on spiked samples in urine ranged from 1.27 to 4.20% and 0.12 to 0.24%, respectively, while inter-assay accuracy and precision ranged from 1.60 to 4.00% and 0.14 to 0.25%, respectively. The mean recovery of lomefloxacin from plasma and urine was 98.34 and 98.43%, respectively. The method was successfully applied to the determination of lomefloxacin in pharmaceuticals and biological fluids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Anti-Infective Agents / analysis*
  • Anti-Infective Agents / blood
  • Anti-Infective Agents / urine
  • Fluoroquinolones / analysis*
  • Fluoroquinolones / blood
  • Fluoroquinolones / urine
  • Humans
  • Linear Models
  • Ophthalmic Solutions / analysis
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / economics
  • Spectrometry, Fluorescence / methods*
  • Time Factors

Substances

  • Anti-Infective Agents
  • Fluoroquinolones
  • Ophthalmic Solutions
  • lomefloxacin