Slice-scan method (SS) scans the two-way chromatographic data at each retention time point, and hence obtains the corresponding spectral "slice"s. Then, the spectral slices are transformed into new spectral profiles by spectral transformation, based on which qualitative and quantitative analysis of the overlapping chromatographic peaks could be done. In this work, a new strategy for directly determining the component of interest in the overlapping chromatographic peak is presented, which takes advantage of the selective spectral slices of the determined component and establishes the best calibration curve by applying the new generated spectral profiles after spectral transformation. Meanwhile, the contents of four bioactive lignans, schisandrin, schisantherin, deoxyschizandrin and gamma-schizandrin in Schisandra chinensis Baill., a famous herb in China were determined using SS together with the new quantitative strategy, and satisfactory results have been obtained.