Colorimetric sensing by using allosteric-DNAzyme-coupled rolling circle amplification and a peptide nucleic acid-organic dye probe

Angew Chem Int Ed Engl. 2009;48(19):3512-5. doi: 10.1002/anie.200805966.


Target detection by the naked eye: The action of an RNA-cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single-stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex-binding dye, the color of the dye changed from blue to purple.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allosteric Regulation
  • Biosensing Techniques*
  • Colorimetry / methods*
  • Coloring Agents / metabolism*
  • DNA Replication
  • DNA, Catalytic / chemistry*
  • DNA, Single-Stranded / biosynthesis*
  • DNA, Single-Stranded / chemistry
  • Nucleic Acid Hybridization
  • Peptide Nucleic Acids / chemistry*


  • Coloring Agents
  • DNA, Catalytic
  • DNA, Single-Stranded
  • Peptide Nucleic Acids