Efficiency of peptides and lipopeptides for in vivo priming of virus-specific cytotoxic T cells

Eur J Immunol. 1991 Nov;21(11):2649-54. doi: 10.1002/eji.1830211102.


Synthetic peptides and a novel type of lipopeptide vaccine, both containing T cell epitopes recognized by Kd-restricted, influenza virus-specific cytotoxic T cells (CTL) were compared in their efficiency to induce virus-specific CTL in vivo. All attempts to induce virus-specific CTL with synthetic peptide (in the absence of adjuvants) failed. However, a latent immunization was observed, resulting in an increased response to the injected peptide seen only after boosting the recipients with immunogenic virus. In contrast, priming with synthetic lipopeptide vaccine (tripalmitoyl-S-glycerylcysteinyl-seryl-serine [P3CSS] coupled to peptide) was successful under most conditions, and matched the priming efficiency seen with infectious virus. The requirements for in vivo priming of virus-specific CTL using lipopeptide suggest that attachment of the lipopeptide to a hydrophobic entity, such as the cell membrane, is responsible for its efficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / immunology
  • Cytotoxicity, Immunologic
  • Immunity, Cellular
  • Immunologic Memory
  • Influenza A virus / immunology
  • Lipoproteins / immunology*
  • Mice
  • Mice, Inbred Strains
  • Nucleocapsid Proteins
  • Nucleoproteins*
  • Peptides / immunology*
  • Structure-Activity Relationship
  • T-Lymphocytes, Cytotoxic / immunology*
  • Time Factors
  • Viral Core Proteins / immunology


  • Antigens, Viral
  • Lipoproteins
  • Nucleocapsid Proteins
  • Nucleoproteins
  • Peptides
  • Viral Core Proteins