Reliable identification of clinically prevalent species and subspecies of staphylococci by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis

Diagn Microbiol Infect Dis. 2009 May;64(1):1-5. doi: 10.1016/j.diagmicrobio.2008.12.017.

Abstract

We identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 10 species and 5 subspecies of Staphylococcus among 139 clinical isolates and compared it with conventional tests. All isolates showed species-specific whole-cell protein profiles, even atypical strains, with up to 60% and at least 73.5% of interspecies and intraspecies similarity, respectively. Except for 5 isolates that presented biochemical profiles of Staphylococcus hominis subsp. hominis, but were identified as S. hominis subsp. novobiosepticus by SDS-PAGE, there was 100% accordance between the methods used. Comparison with the partial 16S-rDNA sequences confirmed by SDS-PAGE showed the high accuracy of this method to identify staphylococci subspecies and species.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / analysis*
  • Bacterial Proteins / isolation & purification
  • Bacterial Typing Techniques / methods
  • Cluster Analysis
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Humans
  • Proteome / analysis*
  • Proteome / isolation & purification
  • Sensitivity and Specificity
  • Staphylococcal Infections / microbiology*
  • Staphylococcus / chemistry*
  • Staphylococcus / classification*
  • Staphylococcus / isolation & purification

Substances

  • Bacterial Proteins
  • Proteome