Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction

Diagn Microbiol Infect Dis. 2009 May;64(1):98-101. doi: 10.1016/j.diagmicrobio.2009.01.031.

Abstract

We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1, stx2, and eae, without the use of probes. The method, which was validated on a collection of 143 EHEC strains, is simple, rapid, cost-effective, and sensitive.

Publication types

  • Evaluation Study

MeSH terms

  • Adhesins, Bacterial / genetics*
  • Benzothiazoles
  • Child
  • Diamines
  • Enterohemorrhagic Escherichia coli / genetics*
  • Escherichia coli Infections / microbiology*
  • Escherichia coli Proteins / genetics*
  • Feces / microbiology
  • Humans
  • Organic Chemicals / metabolism*
  • Polymerase Chain Reaction / methods*
  • Quinolines
  • Shiga Toxin 1 / genetics*
  • Shiga Toxin 2 / genetics*
  • Staining and Labeling
  • Virulence Factors / genetics

Substances

  • Adhesins, Bacterial
  • Benzothiazoles
  • Diamines
  • Escherichia coli Proteins
  • Organic Chemicals
  • Quinolines
  • Shiga Toxin 1
  • Shiga Toxin 2
  • Virulence Factors
  • eaeA protein, E coli
  • SYBR Green I