The cis element and factors required for condensin recruitment to chromosomes

Mol Cell. 2009 Apr 10;34(1):26-35. doi: 10.1016/j.molcel.2009.02.021.

Abstract

Condensins are required for segregation of rDNA repeats in concert with Fob1, a replication fork block protein binding at the replication fork barrier (RFB) site within rDNA in yeast. Here, we found that the RFB site functions as a cis element for Fob1-dependent condensin recruitment onto chromosomes. Replication fork blockage itself is not necessary for condensin recruitment. Instead, by genetic screening, we identified three genes, TOF2, CSM1, and LRS4, required both for condensin recruitment to the RFB site and for assuring the segregation of rDNA repeats. Hierarchical binding of Fob1, these three proteins and condensin, and interactions between Csm1/Lrs4 and multiple subunits of condensin were observed. These results suggest that three proteins control protein interactions linking between Fob1 and condensin, and contribute to ensuring the faithful segregation of rDNA repeats. Our study also suggests that recruitment of condensin onto chromosomes requires cis elements and recruiters that physically interact with condensin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Carrier Proteins / genetics
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cell Cycle Proteins / physiology
  • Chromosomes, Fungal / metabolism*
  • DNA Replication / physiology
  • DNA, Ribosomal / metabolism
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / physiology
  • Intracellular Signaling Peptides and Proteins
  • Multiprotein Complexes / metabolism*
  • Mutation
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Nuclear Proteins / physiology
  • Repetitive Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / physiology

Substances

  • CSM1 protein, S cerevisiae
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • FOB1 protein, S cerevisiae
  • Intracellular Signaling Peptides and Proteins
  • LRS4 protein, S cerevisiae
  • Multiprotein Complexes
  • Nuclear Proteins
  • SMC2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • TOF2 protein, S cerevisiae
  • condensin complexes
  • Adenosine Triphosphatases