Embryonic stem cell-specific microRNAs promote induced pluripotency

Nat Biotechnol. 2009 May;27(5):459-61. doi: 10.1038/nbt.1535. Epub 2009 Apr 12.

Abstract

This report demonstrates that introduction of microRNAs (miRNAs) specific to embryonic stem cells enhances the production of mouse induced pluripotent stem (iPS) cells. The miRNAs miR-291-3p, miR-294 and miR-295 increase the efficiency of reprogramming by Oct4, Sox2 and Klf4, but not by these factors plus cMyc. cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Dedifferentiation*
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism*
  • Mice
  • MicroRNAs / metabolism*
  • Pluripotent Stem Cells / metabolism
  • Proto-Oncogene Proteins c-myc / metabolism

Substances

  • MicroRNAs
  • Proto-Oncogene Proteins c-myc