Expression of B-cell activating factor enhances protective immunity of a vaccine against Pseudomonas aeruginosa

Infect Immun. 2009 Jul;77(7):3044-55. doi: 10.1128/IAI.00927-08. Epub 2009 Apr 13.


B-cell activating factor (BAFF), a member of the TNF family, is a potent cytokine with stimulatory effects on B and T cells. To evaluate the potential of transient overexpression of BAFF to enhance vaccine immunogenicity, a replication-deficient adenovirus expressing full-length murine BAFF (AdBAFF) was tested in a mouse vaccine model against Pseudomonas aeruginosa. When coadministered with heat-killed P. aeruginosa, AdBAFF mediated a significant increase in anti-P. aeruginosa-specific serum and lung mucosal antibodies and resulted in improved protection against a lethal respiratory challenge with P. aeruginosa. This effect was independent of the site of administration of AdBAFF and was observed both when AdBAFF was given simultaneously with heat-killed P. aeruginosa as well as when AdBAFF was administered 4 weeks after immunization with heat-killed P. aeruginosa. These data demonstrate that a temporal increase in systemic BAFF levels is able to augment a P. aeruginosa-specific immune response upon immunization with heat-killed P. aeruginosa, suggesting that the immune-stimulatory effects of BAFF may be exploited as a molecular adjuvant for genetic vaccines.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adjuvants, Immunologic / genetics
  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibodies, Bacterial / analysis
  • Antibodies, Bacterial / blood
  • B-Cell Activating Factor / genetics
  • B-Cell Activating Factor / pharmacology*
  • Female
  • Genetic Vectors
  • Immunity, Mucosal
  • Mice
  • Mice, Inbred C57BL
  • Pneumonia, Bacterial / immunology
  • Pseudomonas Vaccines / immunology*
  • Pseudomonas aeruginosa / immunology*
  • Survival Analysis
  • Vaccines, Inactivated / immunology


  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • B-Cell Activating Factor
  • Pseudomonas Vaccines
  • Vaccines, Inactivated