Characterization of sea urchin egg endoplasmic reticulum in cortical preparations

Dev Biol. 1991 Nov;148(1):398-401. doi: 10.1016/0012-1606(91)90348-7.

Abstract

The cortical endoplasmic reticulum (ER) of sea urchin eggs was localized on isolated egg cortices by staining with aqueous suspensions of the dicarbocyanine "DiI." Immunofluorescence localization of a calsequestrin-like protein was essentially identical; this is consistent with a role for the ER in calcium regulation. The ER often encircles cortical granules, making it well-suited for initiating fusion and propagating the calcium wave. Thiazole orange and Hoechst dye 33258 at pH 2 stain ribosomes bound to the ER, providing evidence that the cortical ER is rough ER. High chloride concentrations were found to disrupt ER continuity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Endoplasmic Reticulum / ultrastructure*
  • Microscopy, Electron
  • Oocytes / ultrastructure*
  • Sea Urchins