Construction by polymerase chain reaction and use of intragenic DNA probes for three main types of transferable beta-lactamases (TEM, SHV, CARB) [corrected]

FEMS Microbiol Lett. 1991 Jul 15;66(1):19-25. doi: 10.1016/0378-1097(91)90414-6.


Intragenic DNA probes were synthesized by polymerase chain reaction using fragments of the genes of three major types of beta-lactamases (TEM, SHV, CARB) as templates. The TEM probe hybridized with the genes encoding TEM-1, TEM-2 and six extended-spectrum related enzymes (TEM-3 to TEM-7, TEM-2O) in colony hybridizations and Southern-blot analysis. The SHV probe hybridized with the genes for SHV-1, OHIO-1 and four derived extended-spectrum beta-lactamases (SHV-2, SHV-3, SHV-4 and SHV-5). The CARB probe hybridized with the genes for PSE-1 (CARB-2), PSE-4 (CARB-1), CARB-3 and CARB-4. None of the probes hybridized with genes for any of eight oxacillin-hydrolysing enzymes, PSE-2, OXA-1 to OXA-7, ROB-1 and chromosomal beta-lactamases of various Enterobacteriaceae (except Klebsiella pneumoniae) and Pseudomonas aeruginosa. Investigations of Escherichia coli clinical isolates using these probes indicate the presence of a novel type of extended-spectrum, transferable beta-lactamase.

MeSH terms

  • Base Sequence
  • DNA Probes*
  • Drug Resistance, Microbial / genetics
  • Genes, Bacterial
  • Gram-Negative Bacteria / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polydeoxyribonucleotides / genetics
  • Polymerase Chain Reaction
  • R Factors
  • beta-Lactamases / classification
  • beta-Lactamases / genetics*


  • DNA Probes
  • Polydeoxyribonucleotides
  • beta-Lactamases