Development of an assay for the detection and quantification of the measles virus nucleoprotein (N) gene using real-time reverse transcriptase PCR

J Med Microbiol. 2009 May;58(Pt 5):638-643. doi: 10.1099/jmm.0.005439-0.


We developed a new quantification method for the measles virus (MeV) nucleoprotein (N) gene using real-time reverse transcriptase PCR. This method allowed us to quantify 10(1)-10(7) copies per reaction (corresponding to 5x10(-1)-5x10(5) copies microl(-1)) of the MeV N gene. We also quantified the MeV N gene from the throat swabs of 22 patients with measles as well as the MeV genotypes A, D3, D5, D9 and H1 in viral suspensions derived from MeV-infected cells. As a result, 3.9x10(3)-5.2x10(6) copies ml(-1) and 7.4x10(7)-2.0x10(8) copies ml(-1) of the MeV genomes (N gene) were detected in the throat swabs and viral suspensions, respectively. No other viruses (enteroviruses, respiratory syncytial virus, human metapneumovirus or mumps virus) were detected in the assay. The results suggest that this method is applicable to the detection and quantification of some genotypes of MeV.

MeSH terms

  • DNA Primers
  • Gene Amplification
  • Genes, Viral / genetics
  • Genotype
  • Humans
  • Measles / virology*
  • Measles virus / genetics*
  • Measles virus / isolation & purification
  • Molecular Sequence Data
  • Nucleocapsid Proteins / genetics*
  • Pharynx / virology
  • Plasmids
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction / methods*


  • DNA Primers
  • Nucleocapsid Proteins
  • RNA, Viral

Associated data

  • GENBANK/AB447494
  • GENBANK/AB447495
  • GENBANK/AB447496
  • GENBANK/AB447497
  • GENBANK/AB447498
  • GENBANK/AB447499
  • GENBANK/AB447500
  • GENBANK/AB447501
  • GENBANK/AB447502
  • GENBANK/AB447503
  • GENBANK/AB447504
  • GENBANK/AB447505
  • GENBANK/AB447506
  • GENBANK/AB447507
  • GENBANK/AB447508
  • GENBANK/AB447509
  • GENBANK/AB447510
  • GENBANK/AB447511
  • GENBANK/AB447512
  • GENBANK/AB447513
  • GENBANK/AB447514
  • GENBANK/AB447515