Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy

Adv Drug Deliv Rev. 2009 Jun 21;61(6):428-37. doi: 10.1016/j.addr.2009.03.009. Epub 2009 Apr 17.


Proteins bind the surfaces of nanoparticles, and biological materials in general, immediately upon introduction of the materials into a physiological environment. The further biological response of the body is influenced by the nanoparticle-protein complex. The nanoparticle's composition and surface chemistry dictate the extent and specificity of protein binding. Protein binding is one of the key elements that affects biodistribution of the nanoparticles throughout the body. Here we review recent research on nanoparticle physicochemical properties important for protein binding, techniques for isolation and identification of nanoparticle-bound proteins, and how these proteins can influence particle biodistribution and biocompatibility. Understanding the nanoparticle-protein complex is necessary for control and manipulation of protein binding, and allows for improved engineering of nanoparticles with favorable bioavailability and biodistribution.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Blood Proteins / analysis
  • Blood Proteins / chemistry
  • Blood Proteins / metabolism*
  • Drug Carriers / metabolism
  • Drug Carriers / pharmacokinetics*
  • Drug Carriers / therapeutic use*
  • Humans
  • Kinetics
  • Nanoparticles / chemistry*
  • Protein Binding / physiology
  • Tissue Distribution


  • Blood Proteins
  • Drug Carriers