Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12

J Bacteriol. 2009 Jun;191(12):4025-9. doi: 10.1128/JB.00118-09. Epub 2009 Apr 17.


The genome of an Escherichia coli MC4100 strain with a lambda placMu50 fusion revealed numerous regulatory differences from MG1655, including one that arose during laboratory storage. The 194 mutational differences between MC4100(MuLac) and other K-12 sequences were mostly allocated to specific lineages, indicating the considerable mutational divergence between K-12 strains.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Escherichia coli / chemistry
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Bacterial*
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Mutation*
  • Recombination, Genetic*
  • Sequence Alignment
  • Sequence Analysis, DNA

Associated data

  • GENBANK/CP001396