Profiling genome-wide histone modifications and variants by ChIP-chip on tiling microarrays in S. cerevisiae

Methods Mol Biol. 2009;543:267-79. doi: 10.1007/978-1-60327-015-1_18.

Abstract

Chromatin immunoprecipitation coupled to DNA microarray has become a widely used method to study transcription factors and chromatin structure. Here, we provide a detailed protocol for the localization of the variant histone Htz1 in the S. cerevisiae genome. This protocol can easily be adapted to fit other purposes such as profiling histone modifications.

MeSH terms

  • Chromatin / metabolism
  • Chromatin Immunoprecipitation / methods*
  • Cross-Linking Reagents / pharmacology
  • DNA, Fungal / isolation & purification
  • Genome, Fungal / genetics*
  • Histones / metabolism*
  • Mutation / genetics*
  • Oligonucleotide Array Sequence Analysis*
  • Polymerase Chain Reaction
  • Protein Processing, Post-Translational* / drug effects
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Solubility / drug effects

Substances

  • Chromatin
  • Cross-Linking Reagents
  • DNA, Fungal
  • Histones