Generation of retinal cells from mouse and human induced pluripotent stem cells

Neurosci Lett. 2009 Jul 24;458(3):126-31. doi: 10.1016/j.neulet.2009.04.035. Epub 2009 Apr 18.


We previously reported a technique for generating retinal pigment epithelia (RPE) and putative photoreceptors from embryonic stem (ES) cells. Here we tested whether our procedure can promote retinal differentiation of mouse and human induced pluripotent stem cells (iPSCs). Treating iPSCs with Wnt and Nodal antagonists in suspension culture induced expression of markers of retinal progenitor cells and generated RPE cells. Subsequently, treatment with retinoic acid and taurine generated cells positive for photoreceptor markers in all but one human cell lines. We propose that iPSCs can be induced to differentiate into retinal cells which have a possibility to be used as patient-specific donor cells for transplantation therapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Differentiation / physiology*
  • Cell Line
  • Embryonic Stem Cells / cytology
  • Fibroblasts / cytology
  • Humans
  • Mice
  • Octamer Transcription Factor-3 / genetics
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / physiology
  • Pluripotent Stem Cells / transplantation
  • Retina / cytology*
  • Retina / physiology*
  • Retinal Degeneration / surgery
  • Retinal Pigment Epithelium / cytology
  • Retinal Pigment Epithelium / transplantation
  • Skin / cytology
  • Transfection


  • Octamer Transcription Factor-3