Autoantibodies against cancer antigens

Methods Mol Biol. 2009;520:11-9. doi: 10.1007/978-1-60327-811-9_2.

Abstract

With the identification of tumor antigens, the host immune response to various types of cancers can now be studied with a high degree of specificity in large cohorts of patients, in the hope of correlating immunity with clinical events and defining immunotherapeutic strategies. Several antigens, such as NY-ESO-1, p53, or SOX2, have attracted attention because of their frequent spontaneous immunogenicity in cancer patients, notably in their ability to induce humoral responses. We describe in this chapter a simple implementation of a serological monitoring platform for autoantibody measurement in cancer patients, from production of recombinant antigens to ELISA testing and interpretation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / immunology*
  • Autoantibodies / immunology*
  • Blood Specimen Collection
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Molecular Biology / methods*

Substances

  • Antigens, Neoplasm
  • Autoantibodies