Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues

David R Kilpatrick; Chen-Fu Yang; Karen Ching; Annelet Vincent; Jane Iber; Ray Campagnoli; Mark Mandelbaum; Lina De; Su-Ju Yang; Allan Nix; Olen M Kew

doi:10.1128/JCM.00702-09

Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues

J Clin Microbiol. 2009 Jun;47(6):1939-41. doi: 10.1128/JCM.00702-09. Epub 2009 Apr 22.

Authors

David R Kilpatrick¹, Chen-Fu Yang, Karen Ching, Annelet Vincent, Jane Iber, Ray Campagnoli, Mark Mandelbaum, Lina De, Su-Ju Yang, Allan Nix, Olen M Kew

Affiliation

¹ Polio and Picornavirus Laboratory Branch, G-10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov

Abstract

We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.

Publication types

Evaluation Study

MeSH terms

DNA Primers / genetics*
Humans
Oligonucleotide Probes / genetics*
Poliovirus / classification*
Poliovirus / isolation & purification*
RNA, Viral / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity

Substances

DNA Primers
Oligonucleotide Probes
RNA, Viral