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. 2009 Apr 22;(26):1098.
doi: 10.3791/1098.

Micro-drive Array for Chronic in Vivo Recording: Tetrode Assembly

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Free PMC article

Micro-drive Array for Chronic in Vivo Recording: Tetrode Assembly

David P Nguyen et al. J Vis Exp. .
Free PMC article

Abstract

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo. The differential amplitude of action potential signatures over the channels of a tetrode allows for the isolation of single-unit activity from multi-unit signals. The ability to precisely control the stereotaxic location and depth of the tetrode is critical for studying coordinated neural activity across brain regions. In combination with a micro-drive array, it is possible to achieve precise placement and stable control of many tetrodes over the course of days to weeks. In this protocol, we demonstrate how to fabricate and condition tetrodes using basic tools and materials, install the tetrodes into a multi-drive tetrode array for chronic in-vivo recording in the rat, make ground wire connections to the micro-drive array, and attach a protective cone onto the micro-drive array in order to protect the tetrodes from physical contact with the environment.

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References

    1. Gray CM, Maldonado PE, Wilson M, McNaughton B. Tetrodes markedly improve the reliability and yield of multiple single-unit isolation from multi-unit recordings in cat striate cortex. J. Neurosci. Methods. 1995;63(1-2):43–54. - PubMed

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