A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase

Angew Chem Int Ed Engl. 2009;48(22):4034-7. doi: 10.1002/anie.200805693.

Abstract

Fast detection of cellular thiols in aqueous medium was achieved using a newly developed fluorescence probe (see picture). Based on this probe, a high-throughput fluorescence assay for glutathione reductase was developed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Coumarins / chemical synthesis
  • Coumarins / chemistry*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorometry / methods
  • Glutathione Reductase / analysis*
  • Humans
  • Sulfhydryl Compounds / analysis*

Substances

  • Coumarins
  • Fluorescent Dyes
  • Sulfhydryl Compounds
  • Glutathione Reductase