Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2

M Gerard; L Fischer; V Moncollin; J M Chipoulet; P Chambon; J M Egly

Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2

J Biol Chem. 1991 Nov 5;266(31):20940-5.

Authors

M Gerard¹, L Fischer, V Moncollin, J M Chipoulet, P Chambon, J M Egly

Affiliation

¹ Laboratoire de Génétique Moléculaire des Eucaryotes, Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine.

PMID: 1939143

Abstract

A general transcription factor (BTF2) has been purified from HeLa whole cell extract and shown to be absolutely required for the formation of a functional initiation complex. We also demonstrate that this factor binds in solution to RNA polymerase B(II) and to the other general transcription factors BTF1(TFIID), BTF3, and BTF4. These results strongly suggest the existence of multiple interactions between the various components of the multiprotein initiation complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HeLa Cells / chemistry*
Humans
Macromolecular Substances
Molecular Weight
Multiprotein Complexes
RNA Polymerase II / metabolism*
Transcription Factors / chemistry
Transcription Factors / isolation & purification*
Transcription Factors / metabolism
Transcription, Genetic*

Substances

Macromolecular Substances
Multiprotein Complexes
Transcription Factors
RNA Polymerase II