Two different homing pathways involving integrin β7 and E-selectin significantly influence trafficking of CD4 cells to the genital tract following Chlamydia muridarum infection

Am J Reprod Immunol. 2009 Jun;61(6):438-45. doi: 10.1111/j.1600-0897.2009.00704.x. Epub 2009 Apr 22.


Problem: Chlamydia trachomatis causes STI and reproductive dysfunction worldwide which is not preventable with antibiotics. Identifying a population of endocervical T cells to target in vaccine development would enhance efficacy.

Method of study: Trafficking of murine CD4+ lymphocytes to Chlamydia muridarum infected genital tract (GT) tissue in vivo was measured using adoptive transfer studies of fluorescent CD4+ T cells from integrin β7-/- mice or mice which lack E-selectin on endothelial cells.

Results: Murine in vivo migration studies showed that lack of α4β7 or E-selectin significantly reduced trafficking of CD4 T cells to the GT of mice infected with C. muridarum.

Conclusion: CD4+ T cells use at least two different adhesive mechanisms involving an integrin of the mucosal homing pathway and selectin pathway to accumulate in the GT during C. muridarum infection.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Movement / genetics
  • Cell Movement / immunology*
  • Chlamydia Infections / genetics
  • Chlamydia Infections / immunology*
  • Chlamydia Infections / microbiology
  • Chlamydia muridarum / immunology*
  • Chlamydia trachomatis
  • E-Selectin / genetics
  • E-Selectin / immunology*
  • Female
  • Genitalia, Female / immunology*
  • Genitalia, Female / microbiology
  • Integrin beta Chains / genetics
  • Integrin beta Chains / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout


  • E-Selectin
  • Integrin beta Chains
  • integrin beta7