Abstract
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Bacterial / immunology
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Bacterial Toxins / immunology
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Bacterial Toxins / isolation & purification*
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Bacterial Toxins / pharmacology
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Biological Assay / methods*
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Cell Line
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Clostridioides difficile / chemistry*
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Clostridioides difficile / immunology
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Cytotoxicity Tests, Immunologic / methods*
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Cytotoxins / immunology
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Cytotoxins / isolation & purification*
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Cytotoxins / pharmacology
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Sensitivity and Specificity
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Swine
Substances
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Antibodies, Bacterial
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Bacterial Toxins
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Cytotoxins