Abstract
The transmembrane transporter P-glycoprotein (P-gp) encoded by ABCB1, is one major cause for multidrug resistance (MDR). We compared the genomic profile and gene expression pattern of the P-gp positive K562VCR cells with parental P-gp negative K562wt cells. In K562VCR array CGH revealed amplification of ABCB1, ABCB4, ABCB5 and SEMA3D, whereas expression microarrays demonstrated upregulation of stem cell genes (e.g. KIT and HOXB4), anti-apoptotic genes (e.g. IGF1R and CCNG1), and downregulation of pro-apoptotic genes (e.g. CASP4, 6 and 7). Thus, K562VCR cells disclose stem cell characteristics including a range of drug resistance mechanisms possibly attained as a stem cell program switched on en bloc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B / genetics*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
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Chromosome Mapping
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Chromosomes, Human
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Flow Cytometry
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Gene Expression Profiling
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Homeodomain Proteins / genetics
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Humans
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K562 Cells / pathology*
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Leukemia, Erythroblastic, Acute / genetics*
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Oligonucleotide Array Sequence Analysis
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Stem Cell Factor / genetics
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Stem Cells / cytology
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Stem Cells / physiology
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Transcription Factors / genetics
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Up-Regulation
Substances
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ABCB1 protein, human
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ABCB5 protein, human
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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HOXB4 protein, human
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Homeodomain Proteins
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Stem Cell Factor
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Transcription Factors
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multidrug resistance protein 3