Zinc-finger-based artificial transcription factors and their applications

Adv Drug Deliv Rev. 2009 Jul 2;61(7-8):513-26. doi: 10.1016/j.addr.2009.03.012. Epub 2009 Apr 23.


Artificial transcription factors (ATFs) are potentially a powerful molecular tool to modulate endogenous target gene expression in living cells and organisms. To date, many DNA-binding molecules have been developed as the DNA-binding domains for ATFs. Among them, ATFs comprising Cys(2)His(2)-type zinc-finger proteins (ZFPs) as the DNA-binding domain have been extensively explored. The zinc-finger-based ATFs specifically recognize targeting sites in chromosomes and effectively up- and downregulate expression of their target genes not only in vitro, but also in vivo. In this review, after briefly introducing Cys(2)His(2)-type ZFPs, I will review the studies of endogenous human gene regulation by zinc-finger-based ATFs and other applications as well.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA / genetics
  • Gene Expression / genetics
  • Gene Expression / physiology
  • Gene Library
  • Genetic Therapy*
  • Humans
  • Transcription Factors / genetics*
  • Zinc Fingers / genetics*


  • Transcription Factors
  • DNA