OH-2, a hyperdiploid myeloma cell line without an IGH translocation, has a complex translocation juxtaposing MYC near MAFB and the IGK locus

Leuk Res. 2009 Dec;33(12):1670-7. doi: 10.1016/j.leukres.2009.03.001. Epub 2009 Apr 23.


Multiple myeloma can be classified into hyperdiploid (HRD) (with 48-74 chromosomes) and non-hyperdiploid tumors (usually with immunoglobulin heavy chain translocations). The OH-2 human myeloma cell line (HMCL) retains the same HRD genotype as the primary tumor, with extra copies of chromosomes 3, 7, 15, 19, and 21. Both OH-2 and primary cells have a complex secondary translocation in which the IGK 3' enhancer is inserted between MYC and MAFB, resulting in dysregulation of both oncogenes. OH-2 provides a unique example of an HMCL and the corresponding primary tumor that are shown to share the same HRD genotype.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Diploidy
  • Enhancer Elements, Genetic
  • Genes, myc*
  • Genotype
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin kappa-Chains / genetics*
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • MafB Transcription Factor / genetics*
  • Multiple Myeloma / genetics*
  • Multiple Myeloma / pathology
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Translocation, Genetic*


  • Immunoglobulin Heavy Chains
  • Immunoglobulin kappa-Chains
  • MAFB protein, human
  • MafB Transcription Factor
  • RNA, Messenger