Analysis of calvarial bone defects in rats using microcomputed tomography: potential for a novel composite material and a new quantitative measurement

Br J Oral Maxillofac Surg. 2009 Dec;47(8):616-21. doi: 10.1016/j.bjoms.2009.02.010. Epub 2009 Apr 22.


Reconstruction of craniomaxillofacial defects is a challenge for surgeons and has psychological and functional burdens for patients. Undoubtedly, there is a need for improved biomaterials and techniques for craniomaxillofacial reconstruction. We assessed the potential regeneration of bone using three modifications of a novel composite and explored the validity of a new measurement using microcomputed tomography (micro-CT). We placed three different composite samples in calvarial defects in rats and analysed healing with micro-CT. The results showed that polycaprolactone (PCL) with phosphate glass fibre is promising for non-load bearing applications in the craniomaxillofacial region. Also, the new micro-CT measurement of the temporal characterisation of the mineralisation of bone (TCBM) has the potential to evolve into a reliable predictor of bony healing and its quality.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / therapeutic use*
  • Bone Density / physiology
  • Bone Diseases / diagnostic imaging
  • Bone Diseases / surgery*
  • Bone Regeneration / physiology
  • Bone Substitutes / chemistry
  • Bone Substitutes / therapeutic use*
  • Bone Transplantation / diagnostic imaging
  • Bone Transplantation / pathology
  • Calcification, Physiologic / physiology
  • Ceramics / chemistry
  • Ceramics / therapeutic use
  • Glass / chemistry
  • Imaging, Three-Dimensional / methods
  • Phosphates / chemistry
  • Plastic Surgery Procedures / methods
  • Polyesters / chemistry
  • Polyesters / therapeutic use
  • Rats
  • Rats, Wistar
  • Skull / diagnostic imaging
  • Skull / surgery*
  • Time Factors
  • Wound Healing / physiology
  • X-Ray Microtomography* / methods


  • Biocompatible Materials
  • Bone Substitutes
  • Phosphates
  • Polyesters
  • bioactive glass 45S5
  • fiberglass
  • polycaprolactone