Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice

J Microsc. 2009 May;234(2):124-9. doi: 10.1111/j.1365-2818.2009.03153.x.


The effect of peritoneal injection of acridine orange and SYTO 16 in mice was investigated. Images of peritoneal tissues stained with these dyes and obtained through a confocal micro-endoscope are presented. Seventy-five Balb/c mice were split into five groups and given peritoneal injections of dye or saline. The proportions of negative outcomes in each group were compared using confidence intervals and the Fisher's exact statistical test. A statistically significant increase in adverse events due to dye injection was not observed. These data provide an initial investigation into the safety of acridine orange and SYTO 16 for in vivo imaging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acridine Orange / toxicity*
  • Animals
  • Data Interpretation, Statistical
  • Fluorescent Dyes / toxicity*
  • Injections, Intraperitoneal
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Confocal*
  • Organic Chemicals / toxicity*
  • Peritoneum / drug effects*
  • Tissue Distribution


  • Fluorescent Dyes
  • Organic Chemicals
  • Acridine Orange