Effect of bone sialoprotein coating of ceramic and synthetic polymer materials on in vitro osteogenic cell differentiation and in vivo bone formation

J Biomed Mater Res A. 2010 Mar 15;92(4):1461-7. doi: 10.1002/jbm.a.32459.

Abstract

In this study, we addressed whether Bone Sialoprotein (BSP) coating of various substrates could enhance the in vitro osteogenic differentiation and in vivo bone formation capacity of human Bone Marrow Stromal Cells (BMSC). Moreover, we tested whether synthetic polymer-based porous scaffolds, despite the absence of a mineral component, could support ectopic bone formation by human BMSC if coated with BSP. Adsorption of recombinant human BSP on tissue culture-treated polystyrene (TCTP), beta-tricalcium phosphate (Osteologic) or synthetic polymer (Polyactive) substrates was dose dependent, but did not consistently accelerate or enhance in vitro BMSC osteogenic differentiation, as assessed by the mRNA expression of osteoblast-related genes. Similarly, BSP coating of porous beta-tricalcium phosphate scaffolds (Skelite) did not improve the efficiency of bone tissue formation following loading with BMSC and ectopic implantation in nude mice. Finally, Polyactive foams seeded with BMSC did not form bone tissue in the same ectopic assay, even if coated with BSP. We conclude that BSP coating of a variety of substrates is not directly associated with an enhancement of osteoprogenitor cell differentiation in vitro or in vivo, and that presentation of BSP on polymeric materials is not sufficient to prime BMSC functional osteoblastic differentiation in vivo.

Publication types

  • Evaluation Study

MeSH terms

  • Adsorption
  • Adult
  • Animals
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / pharmacology
  • Cell Differentiation / drug effects*
  • Cells, Cultured
  • Ceramics / chemistry*
  • Coated Materials, Biocompatible* / chemistry
  • Coated Materials, Biocompatible* / pharmacology
  • Humans
  • Implants, Experimental
  • Integrin-Binding Sialoprotein
  • Materials Testing
  • Mesenchymal Stem Cell Transplantation
  • Mice
  • Mice, Nude
  • Middle Aged
  • Osteogenesis / drug effects*
  • Osteopontin / genetics
  • Osteopontin / metabolism
  • Polymers* / chemistry
  • Polymers* / pharmacology
  • Sialoglycoproteins / chemistry*
  • Sialoglycoproteins / genetics
  • Sialoglycoproteins / pharmacology*
  • Tissue Scaffolds / chemistry
  • Tumor Protein, Translationally-Controlled 1

Substances

  • Calcium Phosphates
  • Coated Materials, Biocompatible
  • IBSP protein, human
  • Ibsp protein, mouse
  • Integrin-Binding Sialoprotein
  • Polymers
  • Sialoglycoproteins
  • TPT1 protein, human
  • Tpt1 protein, mouse
  • Tumor Protein, Translationally-Controlled 1
  • beta-tricalcium phosphate
  • Osteopontin