High-throughput generation of tagged stable cell lines for proteomic analysis

Proteomics. 2009 May;9(10):2888-91. doi: 10.1002/pmic.200800873.

Abstract

We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line*
  • Cloning, Molecular*
  • Mammals
  • Proteins / analysis*
  • Proteins / isolation & purification
  • Proteins / metabolism
  • Proteomics / methods*
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism

Substances

  • Proteins
  • Recombinant Fusion Proteins