Cytokine Gene Polymorphisms Associated With Rheumatoid Arthritis and Periodontitis in Japanese Adults

J Periodontol. 2009 May;80(5):792-9. doi: 10.1902/jop.2009.080573.


Background: Cytokines play a major role in the pathogenesis of rheumatoid arthritis (RA) and periodontitis. Both diseases were previously shown to be partly influenced by cytokine gene polymorphisms. Therefore, we evaluated whether the distributions of the cytokine genotypes were unique to subjects with both diseases.

Methods: The study subjects consisted of Japanese adults with RA (RA group; n = 153), periodontitis only (P group; n = 117), and healthy individuals (H group; n = 108). Clinical periodontal condition was defined by measurements of probing depth, clinical attachment level, and bleeding on probing. Genomic DNA was isolated from peripheral blood and analyzed for the determination of 16 gene polymorphisms encoding interleukin (IL)-1, -2, -4, -6, and -10, tumor necrosis factor-alpha, and transforming growth factor-beta 1.

Results: The frequency of patients with RA who exhibited periodontitis was 89.5% (RA + P group; n = 137). No significant differences were observed in any of the frequencies of cytokine genotypes and alleles among the subject groups. After adjustment for age, gender, and smoking status, multiple logistic regression analysis revealed a significant difference in the distribution of IL-1B +3954 genotypes between RA + P and P groups (P = 0.006) and between RA + P and H groups (P = 0.008).

Conclusion: Japanese individuals with RA and periodontitis may exhibit different distributions of IL-1B +3954 genotypes than healthy controls and subjects with periodontitis only.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alleles
  • Arthritis, Rheumatoid / complications*
  • Arthritis, Rheumatoid / genetics*
  • Asian Continental Ancestry Group / genetics
  • Case-Control Studies
  • Cytokines / genetics*
  • Female
  • Gene Frequency
  • Humans
  • Interleukins / genetics
  • Japan
  • Logistic Models
  • Male
  • Middle Aged
  • Periodontitis / complications*
  • Periodontitis / genetics*
  • Polymorphism, Single Nucleotide
  • Risk Factors
  • Transforming Growth Factor beta1 / genetics
  • Tumor Necrosis Factor-alpha / genetics


  • Cytokines
  • Interleukins
  • Transforming Growth Factor beta1
  • Tumor Necrosis Factor-alpha