Pancreatic response in healthy dogs fed diets of various fat compositions

Am J Vet Res. 2009 May;70(5):614-8. doi: 10.2460/ajvr.70.5.614.


Objective: To indirectly assess the pancreatic response in healthy dogs that were fed diets of different fat compositions with or without supplemental pancreatic enzymes and medium-chain triglycerides (MCTs).

Animals: 10 healthy adult dogs.

Procedures: Dogs were fed 4 diets once in random order at 1-week intervals; food was withheld from the dogs for > or = 12 hours prior to the feeding of each diet. Diets A and B contained 16% and 5% crude fat, respectively; diet C was composed of diet A with pancreatic enzymes; and diet D was composed of diet B with pancreatic enzymes and MCTs. Serum canine trypsin-like immunoreactivity (cTLI) and canine pancreatic lipase immunoreactivity (cPLI) concentrations were measured before (0 hours) and at 1 to 2 and 6 hours after feeding. Serum gastrin concentration was measured at 0 hours and at 5 to 10 minutes and 1 to 2 hours after feeding. A gastrin assay validation study was performed to confirm accuracy of test results in dogs. Data were analyzed by use of a repeated-measures general ANOVA.

Results: Serum cTLI, cPLI, or gastrin concentrations in the dogs did not differ among the different diets fed, among dogs, or over time. When multiple comparisons were analyzed, diet D caused the least amount of measurable pancreatic response, although this difference was not significant.

Conclusions and clinical relevance: Results did not indicate a significant effect of dietary fat content or addition of supplemental MCT oil or pancreatic enzymes in diets on serum cTLI, cPLI, or gastrin concentrations in healthy dogs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Diet / veterinary*
  • Dietary Fats / administration & dosage*
  • Dietary Supplements*
  • Dogs / physiology*
  • Enzymes / administration & dosage
  • Female
  • Gastrins / blood
  • Lipase / blood
  • Male
  • Pancreas / physiology*
  • Random Allocation
  • Reproducibility of Results
  • Triglycerides / administration & dosage*
  • Trypsin / blood


  • Dietary Fats
  • Enzymes
  • Gastrins
  • Triglycerides
  • Lipase
  • Trypsin