Saccharibacillus kuerlensis sp. nov., isolated from a desert soil

Int J Syst Evol Microbiol. 2009 May;59(Pt 5):953-7. doi: 10.1099/ijs.0.005199-0.

Abstract

A taxonomic study was performed on strain HR1(T), which was isolated from a desert soil sample collected from Xinjiang Province (China). Cells were aerobic, Gram-positive-staining, pink-pigmented, sporulating rods with a single lateral flagellum. The organism can grow at 15-42 degrees C and pH 5.0-10.0, optimally at 30-37 degrees C and pH 6.0-8.0. Growth is inhibited by 6 % NaCl. Analysis of almost-complete 16S rRNA gene sequence revealed that the isolate represents a distinct taxon within the genus Saccharibacillus; Saccharibacillus sacchari LMG 24085(T) was the nearest relative (97.9 % sequence similarity). DNA-DNA hybridization showed 29.6 % genetic relatedness between strain HR1(T) and S. sacchari LMG 24085(T). The major isoprenoid quinone was MK-7 and the predominant fatty acid was anteiso-C(15 : 0) (50.3 %). The G+C content of the DNA was 50.5 mol%. Therefore, based on phenotypic criteria and the phylogenetic position, strain HR1(T) belongs to a previously unidentified species of the genus Saccharibacillus, for which the name Saccharibacillus kuerlensis sp. nov. is proposed. The type strain is HR1(T) (=KCTC 13182(T) =JCM 14865(T) =CGMCC 1.6964(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Desert Climate*
  • Fatty Acids / analysis
  • Gram-Positive Endospore-Forming Rods / classification*
  • Gram-Positive Endospore-Forming Rods / genetics
  • Gram-Positive Endospore-Forming Rods / isolation & purification
  • Gram-Positive Endospore-Forming Rods / physiology
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Species Specificity

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Quinones
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/EU046270