Abstract
Here we describe three Escherichia coli clinical isolates with reduced susceptibility to cefepime. Sequencing of the bla(CMY) genes revealed two novel variants (CMY-33 and -44) with two- to four-amino-acid deletions in the H-10 helix. The deletions were responsible for 12- to 24-fold increases in the MICs of cefepime.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology*
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Cefepime
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Cephalosporins / pharmacology*
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Escherichia coli / drug effects*
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Sequence Homology, Amino Acid
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beta-Lactamases / chemistry
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Cephalosporins
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Cefepime
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beta-lactamase CMY-2
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beta-Lactamases