Defective Interfering Particles With Covalently Linked [+/-]RNA Induce Interferon

Nature. 1977 Apr 28;266(5605):815-9. doi: 10.1038/266815a0.


Defective interfering (DI) particles of vesicular stomatitis virus which contain covalently linked complementary [+]message and [-]anti-message RNA as a single-stranded ribonucleoprotein complex within the particle, are extremely efficient inducers of interferon. A single particle can induce a quantum yield of interferon. A single molecule of double-stranded RNA presumed to form, at least in part, on entry into the cell is thought to induce interferon synthesis. Conventional [-]RNA DI particles with the same polypeptide composition as [+/-]RNA DI particles fail to induce interferon.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Viral
  • Antigen-Antibody Reactions
  • Cell Line
  • Cell Survival
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Defective Viruses / metabolism*
  • Defective Viruses / radiation effects
  • Hot Temperature
  • Interferons / biosynthesis*
  • Nucleic Acid Conformation
  • RNA, Viral / metabolism*
  • Templates, Genetic
  • Ultraviolet Rays
  • Vesicular stomatitis Indiana virus / immunology
  • Vesicular stomatitis Indiana virus / metabolism*
  • Vesicular stomatitis Indiana virus / radiation effects
  • Viral Interference


  • Antibodies, Viral
  • RNA, Viral
  • Dactinomycin
  • Interferons
  • Cycloheximide