Identification of natural MHC class II presented phosphopeptides and tumor-derived MHC class I phospholigands

J Proteome Res. 2009 Jul;8(7):3666-74. doi: 10.1021/pr800937k.


MHC molecules present protein-derived peptides to T lymphocytes. By developing TiO2-based microcentrifugation columns, we identified the first phosphorylated MHC class I ligands from tumor tissue (renal cell carcinoma) and, by comparison to healthy renal tissue, found one Brf1-derived ligand as potentially tumor-associated. We further discovered the first natural phosphorylated MHC class II ligands. They revealed several novel phosphorylation sites of significant transmembrane receptors, such as Frizzled 6, CXCR4 and CD20.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD20 / biosynthesis
  • Carcinoma, Renal Cell / metabolism
  • Cell Line, Tumor
  • Frizzled Receptors / biosynthesis
  • Genes, MHC Class I*
  • Genes, MHC Class II*
  • Histocompatibility Antigens Class I / chemistry*
  • Histocompatibility Antigens Class II / chemistry*
  • Humans
  • Ligands
  • Liver Neoplasms / metabolism
  • Peptides / chemistry*
  • Phosphopeptides / chemistry*
  • Phosphorylation
  • Receptors, CXCR4 / biosynthesis
  • Receptors, G-Protein-Coupled / biosynthesis
  • Titanium / chemistry


  • Antigens, CD20
  • FZD6 protein, human
  • Frizzled Receptors
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Ligands
  • Peptides
  • Phosphopeptides
  • Receptors, CXCR4
  • Receptors, G-Protein-Coupled
  • titanium dioxide
  • Titanium