Anomalous quantitation standard growth curves in a laboratory-developed hepatitis C virus (HCV) RNA quantification assay using the TaqMan HCV analyte-specific reagent

Jeffrey J Germer; Jemee D Kathrotiya; Carrie F Bouse; P Shawn Mitchell; Joseph D C Yao

doi:10.1128/JCM.00028-09

Anomalous quantitation standard growth curves in a laboratory-developed hepatitis C virus (HCV) RNA quantification assay using the TaqMan HCV analyte-specific reagent

J Clin Microbiol. 2009 Jul;47(7):2317-20. doi: 10.1128/JCM.00028-09. Epub 2009 May 6.

Authors

Jeffrey J Germer¹, Jemee D Kathrotiya, Carrie F Bouse, P Shawn Mitchell, Joseph D C Yao

Affiliation

¹ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905-0002, USA.

Abstract

A retrospective examination of quantitation standard growth curves associated with 1,000 unique clinical serum specimens tested by a laboratory-developed TaqMan hepatitis C virus analyte-specific reagent-based assay revealed anomalous growth curves associated with 0.40% (95% confidence interval, 0.11% to 1.00%) of these specimens.

MeSH terms

Diagnostic Errors*
Hepacivirus / genetics
Hepacivirus / isolation & purification*
Humans
Molecular Diagnostic Techniques / methods*
RNA / genetics
RNA / isolation & purification*
Reagent Kits, Diagnostic*
Viral Load / methods*

Substances

Reagent Kits, Diagnostic
RNA