Hepatoprotective and curative properties of Kombucha tea against carbon tetrachloride-induced toxicity

J Microbiol Biotechnol. 2009 Apr;19(4):397-402. doi: 10.4014/jmb.0806.374.


Kombucha tea (KT) is sugared black tea fermented with a symbiotic culture of acetic acid bacteria and yeasts, which is said to be tea fungus. KT is claimed to have various beneficial effects on human health, but there is very little scientific evidence available in the literature. In the present study, KT along with black tea (BT) and black tea manufactured with tea fungus enzymes (enzyme-processed tea, ET) was evaluated for hepatoprotective and curative properties against CCl4-induced toxicity, using male albino rats as an experimental model by analyzing aspartate transaminase, alanine transaminase, and alkaline phosphatase in plasma and malondialdehyde content in plasma and liver tissues. Histopathological analysis of liver tissue was also included. Results showed that BT, ET, and KT have the potential to revert the CCl4-induced hepatotoxicity. Among the three types of teas tried, KT was found to be more efficient than BT and ET. Antioxidant molecules produced during the fermentation period could be the reason for the efficient hepatoprotective and curative properties of KT against CCI4-induced hepatotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetobacter / metabolism
  • Alanine Transaminase / blood
  • Alkaline Phosphatase / blood
  • Animals
  • Aspartate Aminotransferases / blood
  • Carbon Tetrachloride / administration & dosage
  • Carbon Tetrachloride / toxicity*
  • Chemical and Drug Induced Liver Injury
  • Data Interpretation, Statistical
  • Fermentation
  • Liver / pathology*
  • Liver Diseases / diet therapy*
  • Liver Diseases / pathology
  • Male
  • Malondialdehyde / analysis
  • Pichia / metabolism
  • Rats
  • Tea* / chemistry
  • Tea* / microbiology
  • Zygosaccharomyces / metabolism


  • Tea
  • Malondialdehyde
  • Carbon Tetrachloride
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Alkaline Phosphatase