Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma

Oncogene. 2009 Jun 11;28(23):2266-75. doi: 10.1038/onc.2009.76. Epub 2009 Apr 27.


Gliomas are primary brain tumors mainly affecting adults. The cellular origin is unknown. The recent identification of tumor-initiating cells in glioma, which share many similarities with normal neural stem cells, has suggested the cell of origin to be a transformed neural stem cell. In previous studies, using the RCAS/tv-a mouse model, platelet-derived growth factor B (PDGF-B)-induced gliomas have been generated from nestin or glial fibrillary acidic protein-expressing cells, markers of neural stem cells. To investigate if committed glial progenitor cells could be the cell of origin for glioma, we generated the Ctv-a mouse where tumor induction would be restricted to myelinating oligodendrocyte progenitor cells (OPCs) expressing 2',3'-cyclic nucleotide 3'-phosphodiesterase. We showed that PDGF-B transfer to OPCs could induce gliomas with an incidence of 33%. The majority of tumors resembled human WHO grade II oligodendroglioma based on close similarities in histopathology and expression of cellular markers. Thus, with the Ctv-a mouse we have showed that the cell of origin for glioma may be a committed glial progenitor cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
  • Animals
  • Avian Proteins / genetics
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Chickens
  • Genetic Vectors / genetics
  • Glioma / genetics
  • Glioma / metabolism
  • Glioma / pathology*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Immunohistochemistry
  • Mice
  • Mice, Transgenic
  • Neoplasms, Experimental / genetics
  • Neoplasms, Experimental / metabolism
  • Neoplasms, Experimental / pathology*
  • Oligodendroglia / cytology*
  • Oligodendroglia / metabolism
  • Phosphoric Diester Hydrolases / metabolism
  • Platelet-Derived Growth Factor / genetics
  • Platelet-Derived Growth Factor / metabolism
  • Platelet-Derived Growth Factor / pharmacology
  • Proto-Oncogene Proteins c-akt / genetics
  • Proto-Oncogene Proteins c-akt / metabolism
  • Receptor, Platelet-Derived Growth Factor alpha / metabolism
  • Receptors, Virus / genetics
  • Stem Cells / cytology*
  • Stem Cells / metabolism
  • Transfection
  • Vimentin / metabolism
  • ras Proteins / genetics
  • ras Proteins / metabolism


  • Avian Proteins
  • Platelet-Derived Growth Factor
  • Receptors, Virus
  • Tva receptor
  • Vimentin
  • Green Fluorescent Proteins
  • Receptor, Platelet-Derived Growth Factor alpha
  • Proto-Oncogene Proteins c-akt
  • Phosphoric Diester Hydrolases
  • 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
  • CNP protein, human
  • Cnp protein, mouse
  • ras Proteins